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Luminex bead coupling protocol

We use this process to create our own Luminex assays from standard ELISA reagents. A version of this protocol can be found on protocols.io.

Reagents

Minimize the exposure of EDC and S-NHS to air and moisture. Use fresh aliquots for each coupling reaction and discard after use. Pierce sells individually packaged, small amounts of S-NHS that can be used in a single use fashion.

Procedure